Preliminary study: Kleinsche Fields increase energy production in cells
The Kleinsche Fields application has already led to numerous improvements in a wide range of symptoms. Although the exact mechanism is not yet fully known, a direct or indirect effect on mitochondrial function is suspected. Prof. Dr. Brigitte König carried out a preliminary in-vitro study to test the assumption that Kleinsche Fields have an influence on the "power plants" of the cells. The researcher and mitochondrial expert used a cell model with PMA-differentiated THP-1 cells. These cells were examined using various parameters that allow conclusions to be drawn about mitochondrial function.
Interesting facts
In this preliminary study, PMA-differentiated THP-1 cells were used as a common cell model. The pre-treated cells were divided into two samples (aliquots) and incubated either in the presence or absence of Kleinsche Fields at 37 °C and 5 % CO2 for 24 hours.
After the incubation period, the activities
- of mitochondrial dehydrogenases (enzyme activity)
- ATP generation (energy production)
- the membrane potential (stress resistance)
- the mitochondrial mass analyzed
During the 24-hour incubation period, the cells were exposed to the stressors H2O2, valinomycin (causes collapse of the mitochondrial membrane potential) or lipopolysaccharide from Escherichia coli (LPS, leads to mitochondrial damage depending on concentration) and the respective reactions were documented.
preliminary study
as cell model
Kleinsche Fields
diagnostic
Higher enzyme activity despite addition of stressors
Enzyme activity of the mitochondria increases
Overall, it can be seen that mitochondrial enzymes are activated in the cells in the presence of Kleinsche Fields. After 24 hours of cultivation with Kleinsche Fields, the mitochondrial enzyme activities increased by about 20 %. This increase was also achieved after addition of the stressor H2O2. After stimulation with further stressors, the mitochondrial enzyme activity even increased to at least 30 %.
Proven increase in ATP generation
Mitochondria produce more energy
With the use of the Kleinsche Fields technology, mitochondrial ATP generation in the cells rose significantly. Prior cultivation of the THP-1 cells in the presence of the Kleinsche Fields for 24 hours significantly increased the amount of mitochondrial ATP generated, also after stimulation with H₂O₂ and other stressors, by more than 300% compared with cultivation without Kleinsche Fields.
Rising mitochondrial mass despite the addition of stressors
More mitochondria detectable in the cells
The stressor hydrogen peroxide destroys mitochondria in a concentration-dependent manner, which leads to a reduction in their mass. After one day of cultivating the cells with Kleinsche Fields, however, higher mitochondrial masses were found compared with cells without the use of the magnetic field technology. Overall, the results provide indications that Kleinsche Fields can increase mitochondrial mass - even with the addition of stressors.
Mitochondria briefly explained:
The body's own "power plants" provide vital cellular energy
Mitochondria are tiny structures in our cells that play a decisive role in our health and our wellbeing. They are often called the "power plants of the cells", and for good reason: their main function is energy production. To do this, they convert oxygen together with nutrients from our food into a form of energy that our cells can use. This energy is stored in the form of adenosine triphosphate (ATP). Without ATP our cells could not function and we would be unable to carry out any physical or mental activities. They are indispensable for our health:
- Energy production: Mitochondria supply the energy required for all cell functions.
- Metabolism: Mitochondria play a key role in metabolism by converting nutrients into energy.
- Cell health: Mitochondria help to break down harmful substances and to keep the cells healthy.
- Ageing and disease: Impaired mitochondrial function can lead to various diseases and to premature ageing.
The cellular powerhouses ensure that our cells have the energy they need to carry out their tasks. It is therefore important to look after our mitochondria and to support them through a healthy lifestyle.
More stable mitochondrial membrane potential detectable
Treated cells remain more resistant to stress
It was shown that the mitochondrial potential remains stable for longer in the presence of the stressor valinomycin compared with untreated cells. As a rule, at the concentrations used and over the chosen incubation period, valinomycin has a concentration-dependent negative effect on the mitochondrial membrane potential. After one day of cultivating the cells with Kleinsche Fields, however, the mitochondrial membrane potential withstands even higher valinomycin concentrations.
Results of the study at a glance
The results of the preliminary study suggest that Kleinsche Fields make the mitochondria more resistant to stressors. ATP generation in particular is increased by the magnetic field technology and remains significantly higher, even after the addition of stressors, compared with the cells that were cultivated without Kleinsche Fields.
Is the study relevant to everyday life?
Our conclusion of the study
The preliminary study provides initial evidence that Kleinsche Fields can improve mitochondrial function. Medical technology could therefore offer a new way to support cellular health and alleviate age-related and chronic health problems:
By supporting cellular health and the body's own ability to regenerate, Kleinsche Fields could provide an innovative way to improve quality of life and lead to a better sense of well-being.
The preliminary study suggests that Kleinsche Fields could promote natural cell regeneration. This finding opens up exciting possibilities for improving energy levels, sleep quality and general well-being.
The findings that Kleinsche Fields could support cellular health open up new perspectives for the application of medical technology in the prevention and treatment of common health problems such as type II diabetes, cardiovascular disease and circulatory disorders.