In-vitro pilot study:
Kleinsche Fields reduce the "basic stress" of cells
The application of Kleinsche Fields technology has proven itself for years in the treatment of numerous complaints. However, the exact mechanism of action is still not fully understood. Mitochondrial expert Prof. Dr. Brigitte König suspects that Kleinsche Fields could directly or indirectly influence the functions of the mitochondria. To test this, an in-vitro pilot study was carried out in the laboratory. Blood samples were taken from two volunteers - a healthy person and a person with signs of fatigue. Certain cells (PBMC) were isolated from these blood samples and examined with regard to mitochondrial function.
Interesting facts
Mitochondrial function was analyzed based on the following activities:
- mitochondrial dehydrogenases (enzyme activity)
- mitochondrially generated ATP (energy production)
- mitochondrial membrane potential (stress resistance)
- mitochondrial mass and
- PGC-1-alpha expression (new formation).
For the pilot study, blood was taken from two volunteers, a healthy person and a person with symptoms of fatigue, from which peripheral blood leukocytes (PBMC) were isolated. The isolated PBMC were divided into two samples (aliquots). Each sample was incubated either in the absence or presence of Kleinsche Fields pads at 37 °C under 5 % CO2. Cultivation was carried out for up to 28 days. After the respective incubation times, the PBMC of the corresponding sample were incubated either with H2O2, with valinomycin, or with lipopolysaccharide of Escherichia coli (LPS) for another 24 hours.
pilot study
blood leukocytes
Kleinsche Fields
diagnostics
Higher enzyme activity despite addition of stressors
Increase in mitochondrial enzyme activity
Overall, the subject with signs of fatigue shows increased mitochondrial enzyme activity after 7 days of cultivation, by about 30%. The results also show that Kleinsche Fields can increase enzyme activity even after the addition of various stressors. Mitochondrial enzyme activity rises by 35% after the addition of the stressor H₂O₂.
After 21 days of cultivation with Kleinsche Fields:
The level of mitochondrial enzyme activity is comparable to cultivation in the absence of the magnetic fields, which indicates a long-term metabolic adaptation and regulation of the mitochondria.
Increase in ATP generation detectable
Energy production in the cells increases
The results of the examination of the subject with signs of fatigue show that Kleinsche Fields can significantly increase or maintain the mitochondrial ATP level. The mitochondrial ATP level rose after 7 days of cultivation by more than 300%, regardless of which stressor (H₂O₂, valinomycin or LPS) the cells were exposed to. The increased ATP generation was still measurable after 21 days of cultivation with Kleinsche Fields.
Demonstrably optimised PGC-1-alpha expression
"Basic stress" of the cells decreases
The cells of the subject with signs of fatigue that were cultivated without Kleinsche Fields expressed significantly more PGC-1-alpha than the cells cultivated with Kleinsche Fields - after 7 days of cultivation 8 times more, and after 21 days approximately 60 times more. This indicates a higher level of stress in the cells without the Kleinsche Fields technology. With increasing addition of the stressor H₂O₂, the cells without Kleinsche Fields also showed a reduced ability to express the important protein PGC-1-alpha. With Kleinsche Fields, the cells had a greater capacity to counteract the H₂O₂ stress with a new synthesis of PGC-1-alpha. This suggests that Kleinsche Fields could regulate and optimise PGC-1-alpha expression. This in turn could lead to a more effective formation of new mitochondria.
Key protein for our health
The importance of PGC-1-alpha
PGC-1-alpha is an indispensable protein that fulfills many important functions in our body. It plays a central role in energy metabolism and the formation of new mitochondria, which are also known as the "power plants" of our cells. The most important functions of the protein are
- Energy production: PGC-1-alpha helps to increase the number and efficiency of mitochondria, leading to improved energy production.
- Metabolism: It plays a role in regulating glucose and fat metabolism, which is important for energy supply and health.
- Thermogenesis: PGC-1-alpha supports heat production (thermogenesis) in brown adipose tissue.
- Cell health: It protects cells from oxidative stress and promotes the formation of new blood vessels.
- Environment: The protein contributes significantly to cell health and adaptation to various environmental conditions.
Increasing mitochondrial number despite the addition of stressors
Increase in mitochondrial mass
The stressor hydrogen peroxide (H₂O₂) destroys mitochondria in a concentration-dependent manner, which leads to a reduction in their mass. After 21 days, however, mitochondrial mass increases in the subject with signs of fatigue, even significantly so, under the use of Kleinsche Fields compared with the cells that were cultivated without Kleinsche Fields. This result is a further indication that Kleinsche Fields can make cells more resistant to stressors.
The pilot study also documented the following:
- There were no significant differences in mitochondrial membrane potential between the cells cultured with Kleinsche Fields and those cultured without the special magnetic field.
- Analogous to the subject with signs of fatigue, investigations were also carried out with the isolated PBMC of a person without signs of fatigue ("normal person"). The results were comparable for the individual parameters analyzed and are therefore not listed in detail.
Results of the pilot study at a glance
The results indicate that the cells treated with Kleinsche Fields are more resistant to stressors. In particular, ATP production in the mitochondria remains higher after treatment with stressors compared with the untreated cells.
These results are supported by the analysis of PGC-1-alpha expression. The increased baseline expression of PGC-1-alpha in the peripheral blood leukocytes examined in the absence of Kleinsche Fields indicates a “stress” caused by the culture conditions. The addition of a further stressor (H₂O₂) leads in these cells to an impairment of PGC-1-alpha expression and thus to an impairment of mitochondrial biogenesis. By contrast, peripheral blood leukocytes cultivated with Kleinsche Fields have a lower baseline stress. After the addition of a further stressor (H₂O₂), PGC-1-alpha expression is increased. The increased PGC-1-alpha expression is reflected in a significant increase in mitochondrial mass, which became measurable after 21 days.
Is the pilot study relevant to everyday life?
Our conclusion of the pilot study
With their ability to improve mitochondrial function, Kleinsche Fields could provide an innovative solution to support cellular health and alleviate age-related and chronic health problems:
Overall, the results of the pilot study show the promising potential of Kleinsche Fields to naturally promote and maintain health, well-being and quality of life.
The pilot study provides initial indications that Kleinsche Fields could support the cells' natural ability to regenerate. This means that the special magnetic fields could be used to treat states of exhaustion, sleep problems and generally improve well-being.
The results suggest that Kleinsche Fields could have positive and preventive effects, particularly in the case of type II diabetes, metabolic syndrome, cardiovascular dysfunctions (circulatory disorders), neurodegenerative diseases, sleep disorders and pain sensations.