In-vitro Pilot Study: Fields of Klein Reduce Cells’ “Baseline Stress”
Fields of Klein technology has proven effective for numerous complaints over many years. However, the exact mechanism of action is still not fully understood. Mitochondria expert Prof. Dr. Brigitte König suspects that Fields of Klein may directly or indirectly influence mitochondrial function. To test this, an in-vitro pilot study was carried out in the laboratory. Blood samples were taken from two volunteer participants – a healthy person and a person experiencing fatigue symptoms. Specific cells (PBMC) were isolated from these blood samples and examined with regard to mitochondrial function.
Worth Knowing
Mitochondrial function was analysed based on the following activities:
- mitochondrial dehydrogenases (enzyme activity),
- mitochondrially generated ATP (energy production),
- mitochondrial membrane potential (stress resistance),
- mitochondrial mass, and
- PGC-1-alpha expression (new formation).
For the pilot study, blood was drawn from two volunteer subjects – a healthy person and a person experiencing fatigue symptoms – from which peripheral blood leukocytes (PBMC) were in turn isolated. The isolated PBMC were split into two samples (aliquots). Each sample was incubated at 37°C under 5% CO2 either in the absence or presence of the Fields of Klein pads. Cultivation lasted up to 28 days. After the respective incubation periods, the PBMC of the corresponding sample were incubated for a further 24 hours with either H2O2, valinomycin, or lipopolysaccharide from Escherichia coli (LPS).
Pilot Study
Blood Leukocytes
Fields of Klein
Diagnostics
Higher enzyme activity despite added stressors
Increase in mitochondrial enzyme activity
Overall, the subject with fatigue symptoms showed an increase in mitochondrial enzyme activity of around 30% after 7 days of cultivation. The results also show that Fields of Klein can increase enzyme activity even after the addition of various stressors. Mitochondrial enzyme activity rose by 35% following the addition of the stressor H₂O₂.
After 21 days of cultivation with Fields of Klein:
mitochondrial enzyme activity is comparable to cultivation without the magnetic fields, suggesting a long-term metabolic adaptation and regulation of the mitochondria.
Increase in ATP generation demonstrated
Energy production in cells increases
Results from the subject with fatigue symptoms show that Fields of Klein can significantly increase or maintain mitochondrial ATP levels. Mitochondrial ATP levels rose by more than 300% after 7 days of cultivation, regardless of which stressor (H₂O₂, valinomycin, or LPS) the cells were exposed to. The increased ATP generation was still measurable after 21 days of cultivation with Fields of Klein.
Demonstrably optimised PGC-1-alpha expression
“Baseline stress” of cells decreases
The cells of the subject with fatigue symptoms cultivated without Fields of Klein expressed significantly more PGC-1-alpha compared to the cells cultivated with Fields of Klein – 8 times more after 7 days of cultivation, and about 60 times more after 21 days. This suggests higher cell stress without Fields of Klein technology. With increasing addition of the stressor H₂O₂, the cells without Fields of Klein also showed a reduced ability to express the important protein PGC-1-alpha. With Fields of Klein, cells had a greater capacity to counter H₂O₂ stress through new synthesis of PGC-1-alpha. This suggests that Fields of Klein could regulate and optimise PGC-1-alpha expression, which in turn could enable more effective new formation of mitochondria.
A key protein for our health
The importance of PGC-1-alpha
PGC-1-alpha is an essential protein that fulfils many important functions in our body. It plays a central role in energy metabolism and in the formation of new mitochondria, also known as our cells’ “power plants.” The protein’s main functions are:
- Energy production: PGC-1-alpha helps increase the number and efficiency of mitochondria, leading to improved energy production.
- Metabolism: it plays a role in regulating glucose and fat metabolism, which is important for energy supply and health.
- Thermogenesis: PGC-1-alpha supports heat production (thermogenesis) in brown adipose tissue.
- Cell health: it protects cells against oxidative stress and promotes the formation of new blood vessels.
- Environment: the protein contributes significantly to cell health and adaptation to different environmental conditions.
Increasing mitochondrial number despite added stressors
Increase in mitochondrial mass
The stressor hydrogen peroxide (H₂O₂) destroys mitochondria in a concentration-dependent manner, reducing their mass. After 21 days, however, mitochondrial mass increased significantly in the subject with fatigue symptoms under Fields of Klein compared to cells cultivated without Fields of Klein. This result is a further indication that Fields of Klein can make cells more resistant to stressors.
Also documented in the pilot study:
- There were no significant differences in mitochondrial membrane potential between cells cultivated with and without Fields of Klein.
- Analogous to the subject with fatigue symptoms, examinations were also conducted with isolated PBMC from a person without fatigue symptoms (“normal person”). Results were comparable across the individual parameters analysed and are therefore not listed in detail.
Results of the pilot study at a glance
The results suggest that cells treated with Fields of Klein are more resistant to stressors. In particular, ATP production in the mitochondria remains higher even after treatment with stressors compared to untreated cells.
These results are supported by the analysis of PGC-1-alpha expression. The increased baseline expression of PGC-1-alpha in the peripheral blood leukocytes examined in the absence of Fields of Klein suggests “stress” caused by the culture conditions. The addition of a further stressor (H₂O₂) impairs PGC-1-alpha expression in these cells and thus impairs mitochondrial biogenesis. By contrast, peripheral blood leukocytes cultivated with Fields of Klein show a lower baseline stress. After addition of a further stressor (H₂O₂), PGC-1-alpha expression increases. This increased PGC-1-alpha expression is reflected in a significant increase in mitochondrial mass, measurable after 21 days.
Is the pilot study relevant to everyday life?
Our conclusion on the pilot study
Through their ability to improve mitochondrial function, Fields of Klein could represent an innovative approach to supporting cell health and alleviating age-related and chronic health problems:
Overall, the results of the pilot study show the promising potential of Fields of Klein to naturally promote and maintain health, well-being, and quality of life.
The pilot study provides initial evidence that Fields of Klein could support the cells’ natural regeneration. This could help with states of exhaustion, sleep problems, and generally improving well-being.
The results suggest that Fields of Klein could have positive and preventive effects, particularly for type II diabetes, metabolic syndrome, cardiovascular dysfunction (circulatory disorders), neurodegenerative diseases, as well as sleep disorders and pain sensitivity.